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Axis-Shield Diagnostics polymorphprep density gradient centrifugation
Polymorphprep Density Gradient Centrifugation, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/density+gradient+centrifugation+polymorphprep/polymorphprep+density+gradient/pmc09239160-198-11-15
Average 90 stars, based on 1 article reviews
polymorphprep density gradient centrifugation - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Low production of reactive oxygen species in granulocytes is associated with organ damage in systemic lupus erythematosus
Article Snippet: PMN and peripheral blood mononuclear cells were isolated from heparinised blood of SLE patients by density gradient centrifugation on PolymorphprepTM (Axis-Shield Poc AS, Norway).

Article Title: PD-1 blockade restores impaired function of ex vivo expanded CD8 + T cells and enhances apoptosis in mismatch repair deficient EpCAM + PD-L1 + cancer cells
Article Snippet: PBMCs were separated by density gradient centrifugation using PolymorphprepTM (AXIS-SHIELD PoC AS, Oslo, Norway) cell separation media.

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: Neutrophils were isolated from peripheral blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: In panel two, blood from the normal, OLK and OSCC stages was collected from the angular vein of rats, and neutrophils were isolated from blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.

Isolation:

Article Title: A20 and the noncanonical NF- κ B pathway are key regulators of neutrophil recruitment during fetal ontogeny
Article Snippet: Differentiation of Hoxb8-SCF cells toward neutrophils was allowed by culture for 4 days in differentiation medium consisting of RPMI 1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 20 ng/mL rmG-CSF (Peprotech), and 2% SCF-containing supernatant as described ( ). .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500 g , 30 minutes, room temperature). ..

Article Title: Neisseria meningitidis -Induced Caspase-1 Activation in Human Innate Immune Cells Is LOS-Dependent
Article Snippet: .. Neutrophils were isolated from whole blood by density gradient centrifugation on PolymorphPrep and LymphoPrep reagents (AXIS-SHIELD poC AS, Oslo, Norway), as previously described [ , ]. .. Due to the lack of gene manipulation tools in human primary neutrophils, THP1 cells were used as a proof-of-concept-experimental model. THP1 cells, both wild-type and cells deficient in MyD88 (THP1-defMYD88), NLRP3 (THP1-defNLRP3), or caspase-1 (THP1-defCASP1) (Invivogen, San Diego, CA), were maintained at a cell density of 1 × 10 6 cells/mL in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 50 U/mL penicillin, 50 μ g/mL streptomycin (Thermo Fisher Scientific, Rockford, IL), 100 μ g/mL normocin, and for selection purposes, 200 μ g/mL zeocin, and 100 μ g/mL hygromycin (Invivogen, San Diego, CA) was added according to the manufacturer's recommendation.

Article Title: A20 and the noncanonical NF-κB pathway are key regulators of neutrophil recruitment during fetal ontogeny.
Article Snippet: .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500g, 30 minutes, room temperature). ..

Article Title: The influence of uremic high cystatin C concentration on neutrophil apoptosis and selected neutrophil functions isolated from healthy subjects
Article Snippet: .. PMNs were isolated from heparinized peripheral blood drawn from healthy donors using density gradient centrifugation on Polymorphprep (Axis-Shield PoC As, Norway) at 450 × g at room temperature for 30 min. ..

Centrifugation:

Article Title: A20 and the noncanonical NF- κ B pathway are key regulators of neutrophil recruitment during fetal ontogeny
Article Snippet: Differentiation of Hoxb8-SCF cells toward neutrophils was allowed by culture for 4 days in differentiation medium consisting of RPMI 1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 20 ng/mL rmG-CSF (Peprotech), and 2% SCF-containing supernatant as described ( ). .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500 g , 30 minutes, room temperature). ..

Article Title: A20 and the noncanonical NF-κB pathway are key regulators of neutrophil recruitment during fetal ontogeny.
Article Snippet: .. Human neutrophils were isolated from umbilical cord or peripheral venous blood by layering cells on a Polymorphprep density gradient (AXIS-SHIELD PoC AS) followed by centrifugation (500g, 30 minutes, room temperature). ..

Gradient Centrifugation:

Article Title: Neisseria meningitidis -Induced Caspase-1 Activation in Human Innate Immune Cells Is LOS-Dependent
Article Snippet: .. Neutrophils were isolated from whole blood by density gradient centrifugation on PolymorphPrep and LymphoPrep reagents (AXIS-SHIELD poC AS, Oslo, Norway), as previously described [ , ]. .. Due to the lack of gene manipulation tools in human primary neutrophils, THP1 cells were used as a proof-of-concept-experimental model. THP1 cells, both wild-type and cells deficient in MyD88 (THP1-defMYD88), NLRP3 (THP1-defNLRP3), or caspase-1 (THP1-defCASP1) (Invivogen, San Diego, CA), were maintained at a cell density of 1 × 10 6 cells/mL in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 50 U/mL penicillin, 50 μ g/mL streptomycin (Thermo Fisher Scientific, Rockford, IL), 100 μ g/mL normocin, and for selection purposes, 200 μ g/mL zeocin, and 100 μ g/mL hygromycin (Invivogen, San Diego, CA) was added according to the manufacturer's recommendation.

Article Title: The influence of uremic high cystatin C concentration on neutrophil apoptosis and selected neutrophil functions isolated from healthy subjects
Article Snippet: .. PMNs were isolated from heparinized peripheral blood drawn from healthy donors using density gradient centrifugation on Polymorphprep (Axis-Shield PoC As, Norway) at 450 × g at room temperature for 30 min. ..



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